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M94A2758.TXT
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1994-10-25
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Document 2758
DOCN M94A2758
TI Saliva can show seroconversion.
DT 9412
AU Sangare KA; Koffi AR; Ehouman A; Pasteur Institut, Cote d'Ivoire.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):231 (abstract no. PB0352). Unique
Identifier : AIDSLINE ICA10/94369817
AB OBJECTIVE: To evaluate the ability of saliva to detect seroconversion.
MATERIEL AND METHODS: From a population at risk for HIV infection, 250
consenting adults were determined to be negative by the following
assays: Abbott Recombinant HIV-1/HIV-2, 3rd generation; Enzygnost anti
HIV-1/HIV-2; Vironostika Mixt. All subjects were free to leave the study
at any time. Once a week saliva was collected from each subject with the
Omni-SAL collector (Saliva Diagnostic Systems, Inc.). The specimen was
tested with Abbott Test-Pack and Wellcozyme GACELISA on the day of
collection. In addition a dried blood spot was collected and assayed
according to the procedure in the Wellcozyme GACELISA protocol. If a
positive was obtained with any test, a blood sample was taken the same
day or as soon as possible for a Western Blot analysis. RESULTS: One
individual had confirmed positive saliva and dried blood spot specimens
in the third week of the study. Western Blots, run on serum samples
obtained the next day and in the following weeks for up to two months,
showed a progressive increase in the intensity and number of bands,
starting with only p24 and gp160 and by two months showing, in addition
p17, p31, gp41, p51, p55, p66 and gp120. No individuals had confirmed
positive dried blood spot specimens that were not positive by saliva.
CONCLUSION: Our study shows that saliva is as effective as a dried blood
spot specimen in determining HIV positivity in early seroconversion.
DE Adult Blotting, Western Cohort Studies Comparative Study
Enzyme-Linked Immunosorbent Assay Evaluation Studies Follow-Up Studies
Human HIV Antibodies/*ANALYSIS/BLOOD HIV Seropositivity/*DIAGNOSIS
HIV-1/*IMMUNOLOGY HIV-2/*IMMUNOLOGY Risk Factors Saliva/*IMMUNOLOGY
Salivary Proteins/*ANALYSIS DUPLICATE PUBLICATION MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).